Journal: Advanced Science

Article Title: Mitochondrial CISD1 Modulates Microglial Metabolic Reprogramming to Drive Stress Susceptibility in Mice

doi: 10.1002/advs.202508957

Figure Lengend Snippet: Pharmacological inhibition of CISD1 restores the microglial metabolic phenotype. A) Schematic diagram illustrates the treatment procedure for cultured BV2 cells. B,C) The mRNA (B) and protein (C) expression of CISD1 in vehicle and LPS+ATP group (n = 5–6). D) The mitochondrial stress test kit showing the change of oxygen consumption rate (OCR) in BV2 cells from vehicle, LPS+ATP, vehicle‐NL‐1 and LPS+ATP‐NL‐1 groups (n = 5). E–H) ATP production (E), Basal respiration (F), maximal respiration (G) and reserve capacity (H) in vehicle, LPS+ATP, vehicle‐NL‐1 and LPS+ATP‐NL‐1 groups (n = 5). I) The glycolysis stress test kit showing the change of extracellular acidification rate (ECAR) in BV2 cells from vehicle, LPS+ATP, vehicle‐NL‐1 and LPS+ATP‐NL‐1 groups (n = 5). J,K) Basal glycolysis (J) and maximal glycolysis (K) in vehicle, LPS+ATP, vehicle‐NL‐1 and LPS+ATP‐NL‐1 groups (n = 5). L) Schematic diagram illustrates the treatment procedure for cultured primary microglia cells. M) Representative western blots and analyses of CISD1 in the vehicle and LPS+ATP group (n = 4). N) The glycolysis stress test kit showing the change of extracellular acidification rate (ECAR) in primary microglia cells from vehicle and LPS+ATP, vehicle‐NL‐1 and LPS+ATP‐NL‐1 groups (n = 5). O,P) Basal glycolysis (O) and maximal glycolysis (P) in vehicle, LPS+ATP, vehicle‐NL‐1 and LPS+ATP‐NL‐1 groups (n = 5). Data are presented as the mean ± SEM. Statistical analysis by unpaired Student's t test in (B, C, M) or one‐way ANOVA with Bonferroni's post hoc test (E–H, J, K, O, P). * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: For stimulation, BV2 cells were primed with LPS (200 ng mL −1 , 3 h; MCE, HY‐D1056) followed by ATP (3 mM, 30 min; MCE, HY‐B2176).

Techniques: Inhibition, Cell Culture, Expressing, Western Blot

Journal: Advanced Science

Article Title: Mitochondrial CISD1 Modulates Microglial Metabolic Reprogramming to Drive Stress Susceptibility in Mice

doi: 10.1002/advs.202508957

Figure Lengend Snippet: Pioglitazone treatment restores the microglial metabolic phenotype. A) Schematic diagram of the experimental procedure for primary microglia. B) NAD + /NADH ratio in vehicle, LPS+ATP, vehicle‐pioglitazone and LPS+ATP‐pioglitazone groups (n = 5). C) The activity of complex I in vehicle, LPS+ATP, vehicle‐pioglitazone and LPS+ATP‐pioglitazone groups (n = 5). D) The activity of complex II in vehicle, LPS+ATP, vehicle‐pioglitazone and LPS+ATP‐pioglitazone groups (n = 5). E) Schematic diagram of the experimental procedure for BV2 cell line. F) The mitochondrial stress test kit showing the change of oxygen consumption rate (OCR) in BV2 cells from vehicle, LPS+ATP, vehicle‐pioglitazone and LPS+ATP‐pioglitazone groups (n = 5). G–J) ATP production (G), basal respiration (H), maximal respiration (I) and reserve capacity (J) in vehicle, LPS+ATP, vehicle‐pioglitazone and LPS+ATP‐pioglitazone groups (n = 5). K) The glycolysis stress test kit showing the change of extracellular acidification rate (ECAR) in BV2 cells from vehicle, LPS+ATP, vehicle‐pioglitazone and LPS+ATP‐pioglitazone groups (n = 5). L, M) Basal glycolysis (L) and maximal glycolysis (M) in vehicle, LPS+ATP, vehicle‐pioglitazone and LPS+ATP‐pioglitazone groups (n = 5). Data are presented as the mean ± SEM. Statistical analysis by one‐way ANOVA with Bonferroni's post hoc test (B–D, G–J, L, M). * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: For stimulation, BV2 cells were primed with LPS (200 ng mL −1 , 3 h; MCE, HY‐D1056) followed by ATP (3 mM, 30 min; MCE, HY‐B2176).

Techniques: Activity Assay